Benjamin Stewart

Contact information

Lawrence Livermore National Laboratory

7000 East Avenue, L-452

Livermore, CA 94550

Email: stewart66@llnl.gov

phone: 925 424-2819

 

Education

Post-Doctoral Researcher, Lawrence Livermore National Laboratory, 2009-2012

Ph.D., Toxicology, University of Colorado, 2008

B.S., Molecular Biology, Brigham Young University, 2001

Research interests

Analytical biochemistry, chromatography, mass spectrometry, quantitative metabolism.

 

My research primarily involves the use of accelerator mass spectrometry quantify 14C-labeled tracers in a variety of biological systems.  I am involved in characterization of metabolic fates of xenobiotics, measurement of post-translational and chemical modifications of proteins, and elucidation of metabolite exchange between microorganisms.   My work also involves development of analytical biochemistry methods and application of these methods to problems in basic biochemistry, pharmacology and toxicology.

 

 Most recent publications/Abstracts:

1. Navid A, Ng DM, Stewart BJ, Wong SE, Lightstone FC: Quantitative In Silico analysis of transient metabolism of acetaminophen and associated causes of hepatotoxicity in humans.   In Silico Pharmacology 2013, 1:14.

2. Stewart BJ, Navid A, Kulp KS, Knaack JL, Bench G: D-Lactate production as a function of glucose metabolism in Saccharomyces cerevisiae. Yeast 2013, 30(2):81-91.

3. Thomas AT, Stewart BJ, Ognibene T, Turteltaub KW, Bench G: Directly coupled HPLC-AMS measurement of chemically-modified protein and peptides. Anal Chem 2013, 85(7):3644-3650.

4. Jiao Y, Navid A, Stewart BJ, McKinlay JB, Thelen MP, Pett-Ridge J: Syntrophic metabolism of a co-culture containing Clostridium cellulolyticum and Rhodopseudomonas palustris for hydrogen production. International Journal of Hydrogen Energy 2012, 37(16):11719-11726.

5. Stewart BJ, Bench G, Buchholz BA, Haack KW, Malfatti MA, Ognibene TJ, Turteltaub KW: Accelerator Mass Spectrometry in Pharmaceutical Development. In Mass Spectrometry Handbook. John Wiley & Sons, Inc.; 2012:259-269.

 

Related publications/Abstracts:

1. Buchholz BA, Sarachine Falso MJ, Stewart BJ, Haack KW, Ognibene TJ, Bench G, Salazar Quintero GA, Malfatti MA, Kulp KS, Turteltaub KW et al: Bioanalytics for Human Microdosing. In Encyclopedia of Drug Metabolism and Interactions. John Wiley & Sons, Inc.; 2011.

2. Ronis MJ, Hennings L, Stewart B, Basnakian AG, Apostolov EO, Albano E, Badger TM, Petersen DR: Effects of long-term ethanol administration in a rat total enteral nutrition model of alcoholic liver disease. Am J Physiol Gastrointest Liver Physiol 2011, 300(1):G109-119.

3. Roede JR, Stewart BJ, Petersen DR: 9.26 - Hepatotoxicity of Reactive Aldehydes. In Comprehensive Toxicology (Second Edition). Edited by Editor-in-Chief:  Charlene AM. Oxford: Elsevier; 2010:581-594.

4. Stewart BJ, Navid A, Turteltaub KW, Bench G: Yeast dynamic metabolic flux measurement in nutrient-rich media by HPLC and accelerator mass spectrometry. Anal Chem 2010, 82(23):9812-9817.

5. Sporty J, Lin SJ, Kato M, Ognibene T, Stewart B, Turteltaub K, Bench G: Quantitation of NAD+ biosynthesis from the salvage pathway in Saccharomyces cerevisiae. Yeast 2009, 26(7):363-369.

6. Stewart BJ, Roede JR, Doorn JA, Petersen DR: Lipid aldehyde-mediated cross-linking of apolipoprotein B-100 inhibits secretion from HepG2 cells. Biochim Biophys Acta 2009, 1791(8):772-780.

7. Cheng L, Stewart BJ, You Q, Petersen DR, Ware JA, Piccotti JR, Kawabata TT, Ju C: Covalent binding of the nitroso metabolite of sulfamethoxazole is important in induction of drug-specific T-cell responses in vivo. Mol Pharmacol 2008, 73(6):1769-1775.

8. Roede JR, Stewart BJ, Petersen DR: Decreased expression of peroxiredoxin 6 in a mouse model of ethanol consumption. Free Radic Biol Med 2008, 45(11):1551-1558.

9. Sampey BP, Stewart BJ, Petersen DR: Ethanol-induced modulation of hepatocellular extracellular signal-regulated kinase-1/2 activity via 4-hydroxynonenal. J Biol Chem 2007, 282(3):1925-1937.

10. Stewart BJ, Doorn JA, Petersen DR: Residue-specific adduction of tubulin by 4-hydroxynonenal and 4-oxononenal causes cross-linking and inhibits polymerization. Chem Res Toxicol 2007, 20(8):1111-1119.